100 µm field of view of Unstained cyanobacteria with a shadow induced by the electron flood gun imaged with the helium ion microscope. ORION is able to take high resolution images with large depth of field on biological specimen without staining by using the electron flood gun. ET detector. 30 keV He Ions, 100 µm field of view, 20 degree tilt, 10.8 mm working distance, 0.8 pA.
Image courtesy of Eva Mutunga and Kate Klein, University of the District of Columbia and the National Institute for Standards and Technology.